Theory of RNA-DNA hybridization—I. Stoichiometry at equilibrium

✍ Scribed by John M. Reiner

• Book ID: 104272138
• Publisher: Springer
• Year: 1975
• Tongue: English
• Weight: 886 KB
• Volume: 37
• Category: Article
• ISSN: 1522-9602
• DOI: 10.1016/s0092-8240(75)80031-0

✦ Synopsis

The equations relating hybridized l%IqA to,free I ~A , in the case of simple hybridization, or to ratio of labelled and unlabelled l~IqA in competitive hybridization, are derived. Analysis of the equations shows how hybridization data may be used to infer properties of the distribution of components in an I~NA mixture, or the relation between two distributions in competitive hybridization. A critical examination of the assumptions underlying the equations indicates that some of them may be violated in certain cases, or have no current support, evidential or theoretical. The consequences of such qualifications for the interpretation of hybridization data are indicated.