Theory of regulation by the attenuation mechanism: stochastic model for the attenuation of the Escherichia coli tryptophan operon
β Scribed by Tomohiro Manabe
- Book ID
- 107824456
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 970 KB
- Volume
- 91
- Category
- Article
- ISSN
- 0022-5193
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We constructed plasmid pAtrp46 in which lacZ gene expression is regulated by the attenuator of the Escherichia coli tryptophan (trp) operon. The attenuation of trp, which occurs in the presence of an excess of tryptophan, is reflected by a decrease in the expression of the lacZ gene of pAtrp46 in a
A 273 bp DNA fragment containing the attenuator of the pyrBI operon was inserted into a synthetic cloning site early in the lacZ gene on a plasmid. By this operation the first few codons of lacZ were joined through a linker to the last 39 codons of the open reading frame for the putative pyrB leader