Theoretical study of ribonucleotide reductase mechanism-based inhibition by 2′-azido-2′-deoxyribonucleoside 5′-diphosphates

✍ Scribed by Susana Pereira; Pedro Alexandrino Fernandes; Maria João Ramos

• Publisher: John Wiley and Sons
• Year: 2003
• Tongue: English
• Weight: 414 KB
• Volume: 25
• Category: Article
• ISSN: 0192-8651
• DOI: 10.1002/jcc.10375

✦ Synopsis

Abstract

2′‐Azido‐2′‐deoxyribonucleoside 5′‐diphosphates are mechanism‐based inhibitors of Ribonucleotide Reductase. Considerable effort has been made to elucidate their mechanism of inhibition, which is still controversial and not fully understood. Previous studies have detected the formation of a radical intermediate when the inhibitors interact with the enzyme, and several authors have proposed possible structures for this radical. We have conducted a theoretical study of the possible reactions involved, which allowed us to identify the structure of the new radical among the several proposals. A new reactional path is also proposed that is the most kinetically favored to yield this radical and ultimately inactivate the enzyme. The energetic involved in this mechanism, both for radical formation and radical decay, as well as the calculated Hyperfine Coupling Constants for the radical intermediate, are in agreement with the correspondent experimental values. This mechanistic alternative is fully coherent with remaining experimental data. © 2003 Wiley Periodicals, Inc. J Comput Chem 25: 227–237, 2004