𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Theoretical studies on protein–nucleic acid interactions. II. Hydrogen bonding of amino acid side chains with bases and base pairs of nucleic acids

✍ Scribed by N. Vasant Kumar; Girjesh Govil

Publisher
Wiley (John Wiley & Sons)
Year
1984
Tongue
English
Weight
601 KB
Volume
23
Category
Article
ISSN
0006-3525

No coin nor oath required. For personal study only.

✦ Synopsis

With a view to understanding the role of hydrogen bonds in the recognition of nucleic acids by proteins, hydrogen bonding between the bases and base pairs of nucleic acids and the amino acids (Am, Gln, Asp and Glu, and charged residues Arg+, Glu-, and Asp-) has been studied by a second-order perturbation theory. Binding energies have been calculated for all possible configurations involving a pair of hydrogen bonds between the base (or base pair) and the amino acid residue. Our results show that the hydrogen bonding in these cases has a large contribution from electrostatic interaction. In general, the charged amino acids, compared to the uncharged ones, form more stable complexes with bases or base pairs. The hydrogen-bond energies are an order of magnitude smaller than the Coulombic interaction energies between basic amino acids (Lys+, Arg+, and His+) and the phosphate groups of nucleic acids. The stabilities of the complexes of amino acids Asn, Gln, Asp, and Glu with bases are in the order: G-X > C X > A-X > U-X or T-X, and G . C-X > A -T(U)-X, where X is one of these amino acid residues. It has been shown that Glu-and Asp-can recognize guanine in single-stranded nucleic acids; Arg+ can recognize G a C base pairs from A -T base pairs in doublestranded structures.

📜 SIMILAR VOLUMES

Theoretical studies on protein–nucleic a
Theoretical studies on protein–nucleic acid interactions. III. Stacking of aromatic amino acids with bases and base pairs of nucleic acids
✍ N. Vasant Kumar; Girjesh Govil 📂 Article 📅 1984 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 658 KB

Stacking of aromatic amino acids tryptophan (Trp), tyrosine (Tyr), phenylalanine (Phe), and histidine (His) with bases and base pairs of nucleic acids has been studied. Stacking energies of the amino acid-base (or base pair) complexes have been calculated by second-order perturbation theory. Our res

Theoretical studies on protein–nucleic a
Theoretical studies on protein–nucleic acid interactions. I. Interaction of positively charged amino acids with nucleic acid fragments
✍ N. Vasant Kumar; Girjesh Govil 📂 Article 📅 1984 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 703 KB

Coulombic interactions between the side chains of charged amino acids (Arg + , Lys-, and H i s + ) and negatively charged phosphate groups of nucleic acid fragments have been studied theoretically. Diribose monophosphate and dideoxyribose monophosphate a r e chosen as model systems for single-strand

Interaction of metal ions with nucleic a
Interaction of metal ions with nucleic acids and related compounds. II. Studies on Au(III)-nucleic acid system
✍ C. K. S. Pillai; U. S. Nandi 📂 Article 📅 1978 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 1013 KB

## Abstract The nature of interaction of Au(III) with nucleic acids was studied by using methods such as uv and ir spectrophotometry, viscometry, pH titrations, and melting‐temperature measurements. Au(III) is found to interact slowly with nucleic acids over a period of several hours. The uv spectr