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Theoretical and experimental investigation of chaperone effects on soluble recombinant proteins inEscherichia coli: effect of free DnaK level on temperature-induced recombinant streptokinase production

✍ Scribed by Balaji Balagurunathan; Guhan Jayaraman

Book ID: 107472988
Publisher: Springer Netherlands
Year: 2008
Tongue: English
Weight: 782 KB
Volume: 2
Category: Article
ISSN: 1872-5325
DOI: 10.1007/s11693-009-9021-z

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## Abstract At the laboratory scale, sudden step increases from 30 to 42°C can be readily accomplished when expressing heterologous proteins in heat‐inducible systems. However, for large scale‐cultures only slow ramp‐type increases in temperature are possible due to heat transfer limitations, where