TheMybandAhi-1genes are physically very closely linked on mouse Chromosome 10

✍ Scribed by X. Jiang; L. Villeneuve; C. Turmel; C. A. Kozak; P. Jolicoeur

• Publisher: Springer-Verlag
• Year: 1994
• Tongue: English
• Weight: 810 KB
• Volume: 5
• Category: Article
• ISSN: 0938-8990
• DOI: 10.1007/bf00352344

✦ Synopsis

Ahi-1 has previously been identified as a common helper provirus integration site on mouse Chromosome (Chr) 10 in 16% of Abelson pre-B-cell lymphomas and shown to be closely linked to the Myb protooncogene.

By using long-range restriction mapping, we have mapped the Myb and Ahi-1 regions within a 120-kbp DNA fragment. The Ahi-1 region is located approximately 35 kbp downstream of the Myb gene. A further comfirmation of this finding was obtained by screening a mouse YAC library. The three positive clones obtained contained both the Myb and Ahi-1 gene sequences. To test whether provirus integration in the Ahi-1 region enhances the expression of Myb by a cis-acting mechanism, we have also examined Myb gene expression in A-MuLV-induced pre-B-lymphomas. Our data have revealed that there is no clear evidence for such activation in the tumors we have tested, indicating that provirus insertion in the Ahi-1 region is activating a novel gene, apparently involved in tumor formation.