TheDefH9-iaaM-containing construct efficiently induces parthenocarpy in cucumber
✍ Scribed by Zhimin Yin; Robert Malinowski; Agnieszka Ziółkowska; Hans Sommer; Wojciech Plcader; Stefan Malepszy
- Book ID
- 111490315
- Publisher
- SP Versita
- Year
- 2006
- Tongue
- English
- Weight
- 748 KB
- Volume
- 11
- Category
- Article
- ISSN
- 1425-8153
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Parthenocarpy (seedless fruits) is a desirable trait that has been achieved in many plant cultivars. We generated parthenocarpic cucumber fruits by introducing the chimeric DefH9-iaaM construct into the cucumber genome using an Agrobacterium tumefaciens-mediated protocol. The construct consists of the DefH9 promoter from Antirrhinum majus and the iaaM coding sequence from Pseudomonas syringae. Transgenic plants were obtained from nine independent transformation events: half of these were tetraploid and did not produce seeds following self-pollination, while the remaining half were capable of displaying parthenocarpy in the subsequent reproductive generation. Of the fruits produced by the transgenic lines, 70–90% were parthenocarpic. The segregation of the marker gene in the transgenic T1 progeny indicated single gene inheritance. The seed set in the transgenic lines and their F1 hybrids was lower than in the non-transgenic control plants. Some of the methodological details and the practical significance of the results are discussed.