A 2-μm DNA-based marker recycling system
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Storici, Francesca; Coglievina, Maristella; Bruschi, Carlo V.
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Article
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1999
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John Wiley and Sons
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English
⚖ 312 KB
A molecular FRT (Flp recombinase recognition target)-based cassette system for multiple gene disruption in the yeast Saccharomyces cerevisiae was developed. FRT DNA sequences were designed with different core mutations and subsequently cloned in direct orientation upstream and downstream of a marker