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The Zinc Center Influences the Redox and Thermodynamic Properties of Escherichia coli Thioredoxin 2

✍ Scribed by Hayat El Hajjaji; Mireille Dumoulin; André Matagne; Didier Colau; Goedele Roos; Joris Messens; Jean-Francois Collet


Book ID
116663959
Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
996 KB
Volume
386
Category
Article
ISSN
0022-2836

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We have constructed three mutants in the thioredoxin (trxA) gene changing its catalytic core between Cys-32 and Cys-35. Oligonucleotide-directed mutagenesis was carried out to replace conservative Gly-33 or Pro-34 by leucine, lysine, glutamine, phenylalanine or tryptophane. The mutants were characte