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The X-ray structure of the NAD-dependent 5,10-methylenetetrahydrofolate dehydrogenase from Saccharomyces cerevisiae

✍ Scribed by Arthur F. Monzingo; Andrew Breksa; Stephen Ernst; Dean R. Appling; Jon D. Robertus


Book ID
111753822
Publisher
Cold Spring Harbor Laboratory Press
Year
2000
Tongue
English
Weight
760 KB
Volume
9
Category
Article
ISSN
0961-8368

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Crystallization of the NAD-dependent 5,1
✍ Monzingo, Arthur F.; West, Mary G.; Schelp, Elisabeth; Appling, Dean R.; Robertu πŸ“‚ Article πŸ“… 1996 πŸ› John Wiley and Sons 🌐 English βš– 189 KB

## Saccharomyces cerevisiae possesses three isozymes of 5,lO-methylenetetrahydrofolate dehydrogenase (MTD). The NADdependent enzyme is the first monofunctional form found in eukaryotes. Here we report its crystallization in a form suitable for high-resolution structure. The space group is P4,2,2 w