The VpreB protein of the surrogate light-chain can pair with some μ heavy-chains in the absence of the λ 5 protein

✍ Scribed by Thomas Seidl; Antonius Rolink; Fritz Melchers

• Book ID: 101386225
• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 132 KB
• Volume: 31
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/1521-4141(200107)31:7<1999::aid-immu1999>3.0.co;2-k

✦ Synopsis

Nineteen different ? heavy-chains, seven of them not capable of forming a pre B cell receptor were expressed in Drosophila melanogaster Schneider cells together with either VpreB1, VpreB2, Q 5, or the complete surrogate light-chain to study their interactions in the formation of the pre B cell receptor. The Q 5 protein alone was unable to bind properly to any of the ? heavy-chains, while the VpreB proteins alone formed complexes with five of the ? heavychains. All ? heavy-chains incapable of forming a pre B cell receptor with surrogate lightchain were also incapable of complex formation with VpreB. The possible role of the VpreB/ ? heavy-chain in allelic exclusion of the heavy-chain locus during B cell development is discussed.