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The virion and soluble antigen proteins of variola, monkeypox, and vaccinia viruses

✍ Scribed by Joseph J. Esposito; John F. Obijeski; James H. Nakano

Publisher
John Wiley and Sons
Year
1977
Tongue
English
Weight
962 KB
Volume
1
Category
Article
ISSN
0146-6615

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✦ Synopsis

Abstract

The structural proteins in purified preparations of variola, monkeypox, and vaccinia viruses were separated and compared by using a high resolution SDS‐polyacrylamide gel electrophoresis system. About 30 proteins were resolved for each virus by autoradiography of longitudinally‐sliced gel rods. Although the autoradioelectropherograms of each virus were similar, it was possible to differentiate them by their unique protein pattern in the 30,000 to 40,000 molecular weight region of the gels. A single virion glycoprotein (mol. wt. = 38 × 10^3^) and a virion phosphoprotein (mol. wt. = 12 × 10^3^) were associated with each of the virus preparations.

Cross‐absorbed monospecific immune sera against variola, monkeypox, and vaccinia virus‐infected cells were used in immunodiffusion tests to precipitate radiolabeled, homologous, soluble antigen proteins. The predominant antigen protein associated with each immunospecific precipitate had a molecular weight of approximately 73,000.

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