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The value of Dowex-50 in fractionation of nucleotides from acid soluble pool. A caution

✍ Scribed by Miroslav Bates; Nebojša Avdalović


Book ID
102625673
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
249 KB
Volume
61
Category
Article
ISSN
0003-2697

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✦ Synopsis


Problems were encountered in separating acid soluble nucleotides into four main groups by chromatography on DowexSO(H+) columns. These difficulties derived from the low affinity of ATP, ADP, GDP, GTP, CDP, and CTP for Dowex-56 (H') ion exchanger under mild acid conditions. Thus, under the conditions described, these compounds travel together with uridine compounds. 1. K.4~2, S.. I\ND COMB, D.