A review hris Iicen niiide of the present knowlcdge of the occurrence of glycosittal SillJStnllceS present in the Solnnnccie, nnd sonic iiccount gireri of nicthotis of isoIntioti nnd ticterminntion of tliat foinid in potritocs (soliiniiie).
(2) Using PfiinkucIi's nict~iotI of estrnction, sohnine, 1n.p. %Oo, hns been isoliitecl from sprouted potntoes iiiitl from i t the elkiiloid solnnidine, m.p. ZIO", Iins 1)ceii prepred.
(3) Determinations of the solubility of solnnine in water and in 96% alcohol liovc been ninth nntl its bctinriour nnd tlint of solnnidiuo towards various rcngents described.
(-1) A study of tho nI)sorption spectrum of thc colour produccd by the nction of sulpliuric ncid and for~nnlclel~yde 011 solnuinc nut1 eoliinidinc lins sliown thnt tlic cxtinction coeficient niny be most rentlily deteruiiiied nt II wnve-length of 5300 A. (Zciss photometer filter S.53). A liiienr relntionsliip exists bctseen the concentrntion of solnninc nnd the cstinction coefficient n t this wnve-lengt,li. Using this colour ns tlic tinsis of ZL niethod of detcnninstion, solnninc in nrnonnts grenter tlinn 3 ing. limy lie deterniitd, with IIII iirciiriicy of &5%.
( 5 ) Solanine (ns distinct froin solnnittine) inny IN tleterniined I)y incans of tlie iucrcnse in reducing p o w r occurring on ric:itl Iiytlrolysie. 13y this niethotl not less t h n i i 15 nig. of solnriine niny 1 ) ~ ileterininetl with an nccitriicy of &5:4.
(6) d study lins IJCCII nintlc of fnctors involveit in the estrnctioii ol' solnninc froin potnto tissue, i.e., the nuinher of estriictions ncccssnry and the possibilit,y of li1)ernting morc soliuiiue from tho cells by freezing. In ndditiori the solubility of solniiine. in the nniinonincul solution from ~vliich i t is finnlly precipitnted hiis been determined.
(7) A mctliod for tlctcrniinntioii of soliiiiinc nnd solnnidinc iu the potnto plnut is given. Using this rnctliod the recovery of soliiiiine ndded to minced potiito tissue liiis 1)een fouiicl to be 95%.
Tlie nutliors wish to record their thnnks to Jlcssrs. J. I,j.ons 6;. Co., Ltd., in aliose Lnborntorics this work \viis curried out, for perniission to pulJlisll.