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The usefulness of CHAPS as a non-cytotoxic stabilizing agent in purification of growth factors

✍ Scribed by Yuhsi Matuo; Nozomu Nishi; Yasuyoshi Muguruma; Yoshino Yoshitake; Yoshishige Masuda; Katsuzo Nishikawa; Fumio Wada

Publisher
Springer
Year
1988
Tongue
English
Weight
657 KB
Volume
1
Category
Article
ISSN
0920-9069

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✦ Synopsis

Among several detergents, a zwitterionic detergent, 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate (CHAPS), was found to be least cytotoxic for cultured mammalian cells. CHAPS improved the activity recovery and elution profile of crude and purified fibroblast growth factors (FGFs) during chromatographies. Diluted preparations of FGFs were stabilized by CHAPS against the loss during storage. Amino acid sequence analysis was not disturbed by CHAPS. CHAPS was removable by reversed-phase high-performance liquid chromatography. These results indicate that CHAPS is useful as a non-cytotoxic stabilizing agent in purification of various kinds of bioactive polypeptides.

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