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The use of UV-visible spectroscopy for the determination of hydrophobic interactions between neuropeptides and membrane model systems

✍ Scribed by John K. Young; William H. Graham; Debbie J. Beard; Rickey P. Hicks

Publisher
Wiley (John Wiley & Sons)
Year
1992
Tongue
English
Weight
338 KB
Volume
32
Category
Article
ISSN
0006-3525

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✦ Synopsis

Ultraviolet-visible spectroscopy has been used as a rapid method to evaluate the hydrophobic interactions between a series of cationic and zwitterionic neuropeptides and dipeptides with the hydrophobic core of two membrane model systems; sodium dodecyl sulfate and lysophosphatidylcholine micelles. If a hydrophobic interaction occurs, a 1-nm bathochromic shift is observed in the uv-visible spectrum of the aromatic side chains when going from aqueous solution to a micellar solution. The aromatic residues of substance P, bradykinin, and Des-Argg bradykinin all exhibited the 1-nm bathochromic shift in the presence of sodium dodecyl sulfate while those of Met-enkephalin did not. The opposite effects were observed in the presence of lysophosphatidylcholine micelles.

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