The use of the resonant mirror biosensor to detect point mutations, as demonstrated with synthetic oligonucleotides

✍ Scribed by K. T. Momynaliev; V. M. Govorun; O. Gnedenko; Yu. D. Ivanov; A. I. Archakov

• Publisher: John Wiley and Sons
• Year: 2003
• Tongue: English
• Weight: 113 KB
• Volume: 16
• Category: Article
• ISSN: 0952-3499
• DOI: 10.1002/jmr.580

✦ Synopsis

Abstract

The possibility of using the resonant mirror biosensor to detect point substitutions in oligonucleotides was demonstrated with a fragment of the Helicobacter pylori 23S rRNA gene, point mutations in which are responsible for clarythromycin resistance. Conditions were optimized for the interaction of a probe immobilized on the sensing surface with targets containing various nucleotide substitutions. A probe allowing reliable discrimination of mutant targets was selected. The mismatch position in the probe was shown to affect the kinetic parameters (response) of hybridization with mutant targets, reporting not only the position, but also the character (G or C) of a substitution. Copyright © 2003 John Wiley & Sons, Ltd.