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The use of spun columns in the purification of double-stranded cDNA appropriate for tailing

✍ Scribed by Sueihua Pan; Paul J. Doherty

Publisher: Elsevier Science
Year: 1986
Tongue: English
Weight: 139 KB
Volume: 156
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(86)90257-5

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✦ Synopsis

A reliable estimation of the efficiency of adding homopolymeric tails to double-stranded cDNA molecules by terminal deoxynucleotidyl transferase is extremely important in the construction of cDNA libraries. Appreciable differences in transformation efficiency result when the homopolymer tails to be annealed are of inadequate length. We report here that the use of a Sephadex G-50 spun column to remove oligo(dT) 1 Z-18 frequently coprecipitating in ethanol with the cDNA results in a more dependable estimation of tail lengths.

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