Mutation detection using fluorescent enz
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Jeff J. Babon; Matthew McKenzie; Richard G. H. Cotton
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Article
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1999
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John Wiley and Sons
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English
β 271 KB
Mutation detection techniques are often limited by sensitivity, ease of use and short fragment lengths. Enzyme mismatch cleavage (EMC) is a technique capable of rapidly scanning 1 kbp fragments of DNA for mutations. It relies on the ability of a bacteriophage resolvase enzyme, T4 endonuclease VII, t