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The use of resolvases T4 endonuclease VII and T7 endonuclease I in mutation detection

✍ Scribed by J. J. Babon; M. McKenzie; R. G. H. Cotton


Book ID
111640307
Publisher
Humana Press Inc
Year
2003
Tongue
English
Weight
163 KB
Volume
23
Category
Article
ISSN
1073-6085

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Mutation detection techniques are often limited by sensitivity, ease of use and short fragment lengths. Enzyme mismatch cleavage (EMC) is a technique capable of rapidly scanning 1 kbp fragments of DNA for mutations. It relies on the ability of a bacteriophage resolvase enzyme, T4 endonuclease VII, t