The use of recombinant fusion proteases in the affinity purification of recombinant proteins
β Scribed by Louis E. C. Leong
- Book ID
- 111640060
- Publisher
- Humana Press Inc
- Year
- 1999
- Tongue
- English
- Weight
- 89 KB
- Volume
- 12
- Category
- Article
- ISSN
- 1073-6085
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Prokaryotic expression of polypeptides as fusion proteins with glutathione-S-transferase has recently been reported as a one-step means of purifying recombinant protein. The usefulness of the glutathione-S-transferase/glutathione- agarose system, however, is significantly limited by the frequent syn
## Abstract In this article, recent progress related to the use of different types of polypeptide fusion handles or βtagsβ for the purification of recombinant proteins are critically discussed. In addition, novel aspects of the molecular cassette concept are elaborated, together with areas of poten