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The use of real-time PCR and fluorogenic probes for rapid and accurate genotyping of newborn mice

✍ Scribed by H.J. Hnatyszyn; E.R. Podack; A.K. Young; R. Seivright; G. Spruill; G. Kraus

Book ID
115638425
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
132 KB
Volume
15
Category
Article
ISSN
0890-8508

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## Abstract We describe the use of quantitative PCR (QPCR) to titer recombinant baculoviruses. Custom primers and probe were designed to gp64 and used to calculate a standard curve of QPCR derived titers from dilutions of a previously titrated baculovirus stock. Each dilution was titrated by both p