The use of RAPD technique for the identification and classification ofPisum sativumL. genotypes

✍ Scribed by Petr Samec; Vít Našinec

• Book ID: 104616221
• Publisher: Springer
• Year: 1996
• Tongue: English
• Weight: 441 KB
• Volume: 89
• Category: Article
• ISSN: 0014-2336
• DOI: 10.1007/bf00034610

✦ Synopsis

The genomic DNAs of 42 Pisum sativum genotypes, representing four wild and cultivated subspecies were used as templates in RAPD reactions . Amplification with eight decamer primers generated 149 polymorphic products . Genetic similarities of RAPD profiles were estimated via a coefficient of Jaccard and then the data were processed by cluster analysis (UPGMA) . Each genotype was clearly identified and separated from the others . Our results show that RAPD technology is a rapid, precise and sensitive technique for identification of pea genotypes . However, the phylogenetic relationships within the Pisum sativum, which we tested by bootstrap analysis (Wagner parsimony), must be interpreted with caution .