The use of radioiodinated albumin to measure internal and external vesicle space
✍ Scribed by Rhoda Blostein; Stephen Benderoff
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 241 KB
- Volume
- 84
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A method is given for measuring external cell or vesicle volume and internal vesicle volume using ralI-and 1*51-labeled albumin, respectively. This method has the advantage that this labeled protein can be separated, by acid precipitation, from acid-soluble compounds commonly used in transport studies.
We report the use of 1311-and 1251-labeled serum albumin as a marker of external or internal vesicular volume. The advantage of this method is that the albumin, present in the same medium as acid-soluble components commonly used in transport studies, can then be separated from them by acid precipitation.
Used in conjunction with 3H,0 to measure total space, we measure external space with 1311-labeled albumin and internal space with 1251-labeled albumin. For comparison, [r4C]inulin was also used to measure external space. The preparation used is a suspension of resealed human red cells in which lz51-labeled albumin was incorporated inside during the lysing step.