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The use of poly(ethylene terephthalate)–poly(aniline) composite for trypsin immobilisation

✍ Scribed by S.S. Caramori; K.F. Fernandes

Book ID
103847315
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
677 KB
Volume
28
Category
Article
ISSN
0928-4931

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✦ Synopsis

This paper presents trypsin immobilisation on strips of poly(ethylene terephthalate)-poly(aniline), activated with glutaraldehyde (PET-PANIG) composite. The photomicrography of the material showed changes corresponding to the chemical modifications produced in the steps of synthesis. The immobilisation process was very efficient under optimal conditions (18.6%). The immobilised and free enzyme presented the same pH and temperature optimum. PET-PANIG-trypsin was able to hydrolyse casein, albumin, gelatine, and skimmed milk. Km app value for PET-PANIG-trypsin was very close to Km of the free enzyme for casein. Immobilised trypsin showed higher stability than the free enzyme, with 100% activity after 14 days of storage at 4 °C and 100% operational stability after 4 cycles of use.

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