The use of plant cell vesicles for immobilization of yeast cells producing ethanol

✍ Scribed by Ch. Karbaum; R. Kleine

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 276 KB
• Volume: 11
• Category: Article
• ISSN: 0138-4988
• DOI: 10.1002/abio.370110322

✦ Synopsis

The preparation of immobilized living yeast cells adsorbed into or onto delipided specimens of the dwarf duckweed Wolffia awhiza (Fam. Lemnaceae) is reported. Tbese yeast cell-plant cell conjugates were used for the repeated batch production of ethanol from glucose (143 to 246 g/l) or saccharose (150 g/l). Up to 25 fermentation cycles a t 30°C were performed. The cycle time for complete jubstrste conversion t o ethanol was reduced 10-fold by a 5-fold increase of the yeast cell Wolffia conjugate concentration ( E = 0.08 to E = 0.4):). The corresponding ethanol production was 11.5 to 13.5 vol% and 9 volyo respectively. The reported results on the discontinuous ethanol fermentation with Wolffiu-immobilized yeast cells open the field for their application in continuous ethanol production processes.