𝔖 Bobbio Scriptorium
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The use of phenyl-Sepharose for the affinity purification of proteinases

✍ Scribed by Mark Prescott; Keith Peek; Dallas P. Veitch; Roy M. Daniel

Book ID
116098171
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
552 KB
Volume
26
Category
Article
ISSN
0165-022X

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A continuous system for the recovery and purification of the penicillin acylase from crude extracts by recycling phenyl-Sepharose gel through three agitated vessels with disc filters of stainless steel is presented. The penicillin acylase present in the crude extract was adsorbed into the phenyl-Sep