The use of paper electrophoresis in studying the metabolism of pseudomonas species. I. Apparatus

✍ Scribed by William F. Daniels; R. H. Weaver

• Book ID: 102764392
• Publisher: John Wiley and Sons
• Year: 1962
• Tongue: English
• Weight: 915 KB
• Volume: 4
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260040302

✦ Synopsis

The sandwich paper electrophoresis technique, such as that of Foster ( 1952b), was modified to permit routine examination of as many as 15 samples of materials such as bacterial cultures, simultaneously, and with greater rapidity than with paper chromatography techniques. By the use of borate buffer at pH 9.6 with aniline hydrogen oxalate for development, materials such as glucose, fructose, 2-ketogluconic acid, and 5-ketogluconic acid could be separated in three hours.

* This investigation represents a portion of a dissertation submitted by one of the authors (W. F. D.) in partial fulfillment of the requirements for the Ph.D. degree a t the University of Kentucky.