In this paper, a new approach for the quality assessment of routinely used reversed-phase high performance liquid chromatography columns is presented. A used column is not directly considered deteriorated when changes in retention occur. If attention is paid to the type and magnitude of the changes,
The use of microparticulate guard columns in reverse-phase high-performance liquid chromatography
β Scribed by G. D. Reed; C. R. Loscombe
- Book ID
- 112721883
- Publisher
- Springer
- Year
- 1982
- Tongue
- English
- Weight
- 270 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0009-5893
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Reversed-phase high performance liquid chromatography continues to grow in importance for the analysis of peptides and proteins in biomolecular and pharmaceutical research. The mobile-phase conditions for separation of proteins and peptides are essentially fixed. Most separations of these solutes ar