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The use of metrizamide as a gradient medium for isopycnic separation of rat liver cells

✍ Scribed by Amy C. Munthe-Kaas; Per O. Seglen


Book ID
115904594
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
799 KB
Volume
43
Category
Article
ISSN
0014-5793

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A novel method is described for the separation on a single gradient of the major intracellular organelles of the secretory pathway, the Golgi, the smooth endoplasmic reticulum, and the rough endoplasmic reticulum. Total microsomes were prepared from rat liver by differential centrifugation and resus