The use of ion-exchange resins for studying ion transport in biological systems

✍ Scribed by O.D. Gasko; A.F. Knowles; H.G. Shertzer; E-M. Suolinna; E. Racker

• Publisher: Elsevier Science
• Year: 1976
• Tongue: English
• Weight: 606 KB
• Volume: 72
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(76)90506-6

✦ Synopsis

A new method that involves the use of ion-exchange resins to study ion transport in biological systems is presented. The transport of organic and inorganic ions was studied in a variety of biological systems including intact cells, subcellular particles (Such as mitochondria and plasma membrane vesicles), and reconstituted membrane vesicles. A comparison with such conventional methods as centrifugation and washing, gel filtration, and membrane filtration suggests that the method has certain advantages with respect to reproducibility, reliability, and convenience.

Methods

Zdn-excZzunge K&U. Dowex AGl-X8 (for anion transport) and Dowex AG 5OW-X8 (for cation transport), both 20-50 mesh, were purchased from Bio-Rad Laboratories, Richmond, Calif. The Dowex AGl-X8 was converted in a column from its chloride form, first to the hydroxide form by treatment with 1 N NaOH until no traces of chloride were detectable s7