✦ LIBER ✦
The use of IAA to overcome interspecific hybrid inviability in reciprocal crosses betweenNicotiana tabacumL. andN. repandaWilld.
✍ Scribed by W. M. Zhou; K. Yoshida; Y. Shintaku; G. Takeda
- Book ID
- 104672372
- Publisher
- Springer
- Year
- 1991
- Tongue
- English
- Weight
- 859 KB
- Volume
- 82
- Category
- Article
- ISSN
- 0040-5752
No coin nor oath required. For personal study only.
✦ Synopsis
Flowering hybrid plants were obtained from reciprocal crosses between N. tabacum L. (2n=48) and N. repanda Willd. (2n = 48), in which cross incompatibility and hybrid inviability were manifested. Stylar pollination and ovule culture were used to overcome the cross incompatibility of stylar barriers and ovular death. It was shown that application of 2 mg/l indole-3-acetic acid (IAA) during the growth period, from the fiveto six-leaf stage to the flowering stage, is a useful and easy method to overcome hybrid inviability.