✦ LIBER ✦
The use of high-pressure liquid chromatography in the simultaneous assay of 11β-hydroxylase and 18-hydroxylase in zona fascloulata-retioularis tissue of the rat adrenal cortex
✍ Scribed by Samuel Gallant; Sally M. Bruckheimer; Alexander C. Brownie
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 425 KB
- Volume
- 89
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
An isocratic, reverse-phase system for determining adrenal cortical steroids by high-pressure liquid chromatography is described. The method enables the rapid, accurate, and precise separation and quantitation of the major steroids produced during in vitro incubations of rat adrenal cortex mitochondria with 1 I-deoxycorticosterone.
The application of this method to the determination of lip-and IS-hydroxylase activity in the presence of metopirone is illustrated, and its utility for studying other enzymatic reactions in the adrenal gland is discussed.