𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The use of GC×GC/TOF MS with multivariate analysis for the characterization of foodborne pathogen bacteria profiles

✍ Scribed by J. Yvette Gardner; Darcy E. Brillhart; Melissa M. Benjamin; Lurlene G. Dixon; LaTonya M. Mitchell; Jean-Marie D. Dimandja

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
430 KB
Volume
34
Category
Article
ISSN
1615-9306

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The cellular fatty acid profiles of eight strains of Bacillus, Staphylococcus, and Enterobacteriacae (Escherichia coli and Salmonella) were analyzed by comprehensive two‐dimensional gas chromatography/time‐of‐flight mass spectrometry. A novel template method was developed to standardize the raw two‐dimensional gas chromatography retention data through the use of a chemical indexing mixture. Analyte retention coordinates were normalized in the primary dimension with respect to a series of n‐alkanes (Kovats index) and in the secondary dimension with respect to a series of aromatic hydrocarbons (Lee index). Fatty acid profiles extracted from the templates were compared by multidimensional scaling and principal component analysis. Differences in the profiles of Gram‐positive and Gram‐negative bacteria were observed, and a series of heterogeneous mixtures comprising different fractions (containing one Gram‐positive and one Gram‐negative bacteria strain) were also distinguished from their homogeneous constituents.

📜 SIMILAR VOLUMES

The use of GC-MS-MS for the characteriza
The use of GC-MS-MS for the characterization of naturally occurring methyl alkanes
✍ James F. Carter; Josef Rechka; Neil Robinson 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 191 KB 👁 1 views

TIE cula1y:ji.s of t h e lipid extrac:t.s from samples of photosynthetic; microbial mat.(:. c;ol.lected f ran h o t spr-ings in t h r e e c x c g r a p h i c a l r e g i o n s o f ~~: e J . ~~n d diow~?d t h e p r e s e n c e of a n u r b r of C18-C21 methyl a l k a n e s , To faci 1.i t a t e t,he

The use of multidimensional capillary ch
The use of multidimensional capillary chromatography in conjunction with a triple quadropule mass-spectrometer in the analysis of complex mixtures (GC-GC-MS-MS)
✍ Claude, S. G. ;Tabacchi, R. 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 288 KB 👁 1 views

The analysis of complex mixtures, such as essential oils requires high resolution chromatographic separation and even the use of very efficient columns cannot prevent the overlapping of certain peaks. This means that it is very difficult to obtain high quality mass spectra when the sample contains n

Methodical approach for the use of GC-TO
Methodical approach for the use of GC-TOF MS for screening and confirmation of organic pollutants in environmental water
✍ Tania Portolés; Elena Pitarch; Francisco J. López; Juan V. Sancho; Félix Hernánd 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 345 KB 👁 1 views

## Abstract The potential of gas chromatography coupled to time‐of‐flight mass spectrometry (GC‐TOF MS) for the screening of organic pollutants in water was explored. After a conventional SPE step with C~18~ cartridges, the comparison of spectra with available libraries together with an evaluation

Use of a Thermal Extraction Unit for Fur
Use of a Thermal Extraction Unit for Furnace-type Pyrolysis: Suitability for the Analysis of Polymers by Pyrolysis/GC/MS
✍ Stilianos G. Roussis; Jim W. Fedora 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 690 KB

Taking advantage of the characteristic features of a thermal extraction unit for quantitative analysis, it has been possible to extend its utility to hrnace-type pyrolysis of polymers. Pyrolkis is achieved by accurate temperature programming of the pyrolysis cell from ambient to very high temperatur