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The use of gamma-irradiation and ultraviolet-irradiation in the preparation of human melanoma cells for use in autologous whole-cell vaccines

✍ Scribed by Donna H Deacon; Kevin T Hogan; Erin M Swanson; Kimberly A Chianese-Bullock; Chadrick E Denlinger; Andrea R Czarkowski; Randy S Schrecengost; James W Patterson; Mark W Teague; Craig L Slingluff

Book ID
115011426
Publisher
BioMed Central
Year
2008
Tongue
English
Weight
380 KB
Volume
8
Category
Article
ISSN
1471-2407

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✍ Franloise VessiΓ©re-Louveaux; Martine Darville; Alex Knuth; Thierry Boon πŸ“‚ Article πŸ“… 1985 πŸ› John Wiley and Sons 🌐 French βš– 496 KB πŸ‘ 2 views

## Abstract By using irradiated BALB/c 3T3 mouse fibroblasts as feeder cells, we obtained a marked increase in the cloning efficiency of the 9 human melanoma cell lines that were tested. Four of these melanoma lines had a cloning efficiency that was lower than 0.0007 in the absence of feeder cells.