The use of fluorogenic substrates for measuring enzyme activity in peatlands
โ Scribed by C. Freeman; G. Liska; N. J. Ostle; S. E. Jones; M. A. Lock
- Publisher
- Springer
- Year
- 1995
- Tongue
- English
- Weight
- 494 KB
- Volume
- 175
- Category
- Article
- ISSN
- 0032-079X
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A new fluorogenic substrate, benzyloxycarbonyl+phenylalanine 4-methylcoumaryl-7-ester, has been developed for determination of the esterase activity of ol-chymotrypsin and related enzymes. Synthesis of the substrate was achieved simply by the carbodiimide condensation of benzyloxycarbonyl+phenylalan
An automated method for measurement of proteinase activities using fluorogenic substrates is described. Enzyme assays were performed' in polystyrene microtitration trays as normally used for the enzyme-linked immunosorbent assay technique. The reaction products were measured using an inverted fluore
A new highly sensitive nonseparation enzyme immunoassay for human serum ferritin is described. Reagents include a @-galactosidase-ferritin conjugate, sheep anti-fenitin, anti-sheep IgG, and dextran-linked fl-galactosylumbelliferone as enzyme substrate. The method is based on inhibition of enzyme act
A simple, inexpensive, and sensitive assay for peptidase activity has been devised. The assay was performed in a microtiter plate and was based on fluorogenic peptide substrates, many of which are commercially available. 7-Amino-4-methyl coumarin the fluorescent product liberated during an incubatio