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The use of fluorescent lipid as a non-covalent bound tracer of integral membrane proteins

✍ Scribed by Håkan Eriksson

Book ID
118901127
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
458 KB
Volume
106
Category
Article
ISSN
0022-1759

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✍ Gonzalez-Ros, J. M. ;Calvo-Fernandez, P. ;Šator, V. ;Martinez-Carrion, M. 📂 Article 📅 1979 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 649 KB

Acetylcholine receptor (AcChR) enriched membrane fragments from Torpedo californica electroplax were labeled by in situ photogenerated nitrenes from a hydrophobic fluorescent probe, pyrene-1-sulfonyl azide. Preferential photolabeling of membrane proteins, mainly AcChR, has been achieved and there is