The use of ellipsometry to study adsorption on hydrogels
✍ Scribed by Donald R. Miller; Nikolaos A. Peppas
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 905 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0142-9612
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Protein multilayers were prepared on silica surfaces by up to 20 alternating incubations of a biotin-protein conjugate and polymerized streptavidin. Spectroscopic ellipsometry (350-750 nm) was used to investigate the physical thickness and the dispersion of the protein layer after each incubation st
## Abstract The application of ellipsometry of the study of the adsorption behavior of proteins and synthetic macromolecules at the air‐water interface has been investigated. It is shown that for macromolecules the amount adsorbed per unit area, Γ, as determined by ellipsometry, only has a well‐def