The use of double formvar film to support paraffin sections for light microscopy and subsequent STEM/X-ray microanalysis

✍ Scribed by Tevdt, K�re E. ;Halgunset, Jostein ;Kopstad, Cunnar ;Haugen, Olav A.

• Publisher: Wiley (John Wiley & Sons)
• Year: 1988
• Tongue: English
• Weight: 691 KB
• Volume: 8
• Category: Article
• ISSN: 0741-0581
• DOI: 10.1002/jemt.1060080216

✦ Synopsis

T r o n d h e i m , N o r w a y I n t r o d u c t i o n . T h e p r e s e n t m e t h o d was d e v e l o p e d in o r d e r t o m o r e e f f i c i e n t l y r e c o r d a n d a n a l y s e i n f r e q u e n t l y a p p e a r i n g c r y s t a l l o i d s in t h e a c i n a r lumen o f p r o s t a t i c adenocarcinoma. F o r t h i s p u r p o s e a c r y s t a l l o i d h a d t o b e i d e n t i f i e d by L M -c r i t e r i a a n d l a t e r t h e same p a r t i c l e h a d t o b e a n a l y s e d in t h e EM. However, t h e simple p r o c e d u r e , a n d t h e i n e x p e n s i v e a n d e a s i l y available m a t e r i a l s u s e d , make t h i s a v e r s a t i l e method. M a t e r i a l s a n d Methods. A f o r m v a r film, made f r o m 0.5% F o r m v a r in d i c h l o r e t h a n e a n d c a s t o n d i s t i l l e d w a t e r , i s m o u n t e d o n a g

r a p h i t e r e t a i n e r ( n o g r i d ) w h i c h f i t s t h e JEOL 100 C X specimen h o l d e r ( T v e d t e t al. 1984).

T h e r e t a i n e r i s allowed t o dry in a v e r t i c a l p o s i t i o n in d u s t -f r e e s u r r o u n d i n g s .

A p a r a f f i n s e c t i o n i s c a r e f u l l y p i c k e d up o n t h e F o r m v a r f i l m a n d d r i e d f o r 30 m i n a t 58OC. D e p a r a f f i n i z a t i o n i s p e r f o r m e d w i t h t h e r e t a i n e r in a 40 x 10 mm g l a s s v i a l in w h i c h a tiny hole has b e e n d r i l l e d t h r o u g h t h e b o t t o m ( F i g . 1 ) .

T h i s " l e a k y v i a l " i s s e q u e n t i a l l y