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The use of dinoflagellate bioluminescence to characterize cell stimulation in bioreactors

✍ Scribed by Antony K. Chen; Michael I. Latz; John A. Frangos

Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
327 KB
Volume
83
Category
Article
ISSN
0006-3592

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✦ Synopsis

Abstract

Bioluminescent dinoflagellates are flow‐sensitive marine organisms that produce light emission almost instantaneously upon stimulation by fluid shear in a shear stress dose‐dependent manner. In the present study we tested the hypothesis that monitoring bioluminescence by suspended dinoflagellates can be used as a tool to characterize cellular response to hydrodynamic forces in agitated bioreactors. Specific studies were performed to determine: (1) impeller configurations with minimum cell activation, (2) correlations of cellular response and an integrated shear factor, and (3) the effect of rapid acceleration in agitation. Results indicated that (1) at a volumetric mass transfer coefficient of 3 Γ— 10^βˆ’4^ s^βˆ’1^, marine impeller configurations were less stimulatory than Rushton configurations, (2) bioluminescence response and a modified volumetric integrated shear factor had an excellent correlation, and (3) rapid acceleration in agitation was highly stimulatory, suggesting a profound effect of temporal gradients in shear in increasing cell stimulation. By using bioluminescence stimulation as an indicator of agitation‐induced cell stimulation and/or damage in microcarrier cultures, the present study allows for the verification of hypotheses and development of novel mechanisms of cell damage in bioreactors. Β© 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 83: 93–103, 2003.

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