The use of digital image analysis of chromatin texture in feulgen-stained nuclei to predict recurrence of low grade superficial transitional cell carcinoma of the bladder

✍ Scribed by Roland Van Velthoven; Michel Petein; Willem J. Oosterlinck; Hendrik Roels; Jean-Lambert Pasteels; Claude Schulman; Robert Kiss

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 940 KB
• Volume: 75
• Category: Article
• ISSN: 0008-543X
• DOI: 10.1002/1097-0142(19950115)75:2<560::aid-cncr2820750220>3.0.co;2-b

✦ Synopsis

Background. Identifying a marker enabling prediction of recurrence in the group of superficial transitional cell carcinomas (sTCCs) of the bladder remains an important challenge today. This report quantitatively describes chromatin patterns with respect to such sTCC recurrence.

Materials and Methods. Twenty-nine patients with sTCCs who did not exhibit tumor recurrence within a minimum of 24 months were compared with 21 patients with sTCCs who exhibited tumor recurrence two or three times in a 24-month period, for a total of 74 sTCCs. Quantitative chromatin pattern description was performed by the digital cell image analyses of Feulgen-stained nuclei. Six morphonuclear parameters were thus described and subsequently used to determine a score, allowing biological behavior of sTCCs to be described, i.e., recurrence versus non-recurrence in one calculation step. DNA ploidy level was also determined in each sTCC by assessing its DNA histogram type.

Results. Of 32 patients with Grade 1 pathologically classified pTa/pTl tumors, DNA ploidy level determination permitted correct prediction of tumor nonrecurrence or recurrence of 13 @I%), whereas determination of the