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The use of differential scanning calorimetry (DSC) to determine the correctness of folding of cloned proteins

โœ Scribed by John M. Brewer

Book ID
118713793
Publisher
Portland Press
Year
1999
Tongue
English
Weight
67 KB
Volume
30
Category
Article
ISSN
0885-4513

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โœ D.T. Haynie; E. Freire ๐Ÿ“‚ Article ๐Ÿ“… 1994 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 732 KB

We demonstrate a method for obtaining accurate estimates of the thermodynamic parameter values characteristic of a two-state folding/unfolding transition under conditions in which the onset of cold denaturation prevents the native state from being fully populated at any temperature. This situation o