𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The use of different fixatives and hydrophilic embedding media (Historesin™ and Unicryl™) for the study of embryonic tissues

✍ Scribed by Santander, R. González; Martínez Cuadrado, G.; González-Santander Martínez, M.; Monteagudo, M.; Martínez Alonso, F.J.; Toledo Lobo, M. V.


Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
673 KB
Volume
36
Category
Article
ISSN
1059-910X

No coin nor oath required. For personal study only.

✦ Synopsis


The effects of different fixatives, dehydration procedures, and embedding media on the structural and ultrastructural preservation of young chick embryos (Hamburger and Hamilton stages 18-24) have been studied by means of light and electron microscopy techniques. Under the light microscope, the results obtained with the use of Bouin, glutaraldehyde, or glutaraldehyde-paraformaldehyde mixtures, followed by partial dehydration of the samples and the embedding with two different polar resins (Historesin and Unicryl), were compared with the results obtained using conventional paraffin-embedding methods. Cell and tissue shrinkage was determined by comparing blood cells from those embryos embedded in either of resins with those embedded in paraffin. Samples were also compared with blood smears, either methanol-fixed or unfixed, obtained from embryos at the same Hamburger and Hamilton stages. The results obtained when Unicryl and Araldite were used for electron microscopy have also been compared. When ultrastructural images from glutaraldehyde-tannic acid/osmium tetroxide fixed, Unicryl embedded samples were compared with those from araldite embedded samples, the same good results were observed with either of the resins. Araldite embedding requires a complete dehydration of the samples, while Unicryl allows the embedding of partially dehydrated embryos with optimal ultrastructural results. We suggest that these polar resins can be considered as complementary tools for embedding delicate embryonic tissues, allowing partial dehydration of the specimens with an excellent cell and tissue preservation.


📜 SIMILAR VOLUMES


Nested polymerase chain reaction-based H
✍ Bateman, Adrian C.; Hemmatpour, Shahram K.; Theaker, Jeffrey M.; Howell, W. Mart 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 325 KB 👁 1 views

Human leukocyte antigen (HLA) genotyping is routinely performed prior to organ transplantation using peripheral blood leukocyte-derived DNA. In addition, polymerase chain reaction (PCR)-based methods have permitted HLA genotyping using DNA extracted from formalin-fixed and paraffin-embedded tissue,

The use of sodium sulphide-fixed brain t
✍ J. Mitchell; N. Best; L. E. Sundstrom; H. V. Wheal 📂 Article 📅 1993 🏛 Springer 🌐 English ⚖ 968 KB

The use of sodium sulphide-perfused material for the immunocytochemical demonstration of microglia and astrocytes is described. An intracerebroventricular injection of kainic acid (KA) was used to induce neuronal degeneration and subsequent axonal sprouting in the hippocampus. Animals under deep ana

Differential localization of ErbB2 in di
✍ Nebila Idris; Coralie A. Carothers Carraway; Kermit L. Carraway 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 494 KB

## Abstract ErbB2 has been implicated in numerous functions, including normal and aberrant development of a variety of tissues. Although no soluble ligand has been identified for ErbB2, we have recently shown that ASGP‐2, the transmembrane subunit of the cell surface glycoprotein Muc4 (also called