The use of dietary loading of 133Cs as a potassium substitute in NMR studies of tissues

✍ Scribed by B. Philip Shehan; R. Mark Wellard; William R. Adam; David J. Craik

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 977 KB
• Volume: 30
• Category: Article
• ISSN: 0740-3194
• DOI: 10.1002/mrm.1910300508

✦ Synopsis

Abstract

^133^Cs NMR chemical shifts and relaxation times have been measured for tissue samples in vitro and in vivo from rats which have been fed on a high cesium, low potassium diet, which leads to a predominantly intracellular distribution of this ion, similar to that of K
^+^. The high sensitivity, large chemical shift range, and narrow linewidths of ^133^Cs, compared with ^39^K, allow chemical shift differences to be observed between tissues, and in subcellular organelles such as mitochondria. For example, in vitro tissue chemical shifts, relative to 150 mM CsCl, are 1.06 ± 0.11 ppm for liver, 0.02 ± 0.05 ppm for brain, 1.76 ± 0.20 ppm for erythrocytes, and −0.13 ± 0.02 ppm for plasma. T~1~ and spin‐echo T~2~ values range from 1.26 ± 0.05 s (T~2~), and 0.028 ± 0.006 s (T~2~) for liver, to 6.49 ± 0.19 s and 1.12 ± 0.03 s for plasma. ^133^Cs relaxation times show the same relative trends between tissues as are observed in ^39^K tissue Studies.