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The use of denaturing conditions for gel diffusion of insoluble epidermal proteins

✍ Scribed by Loretta D. Lee; Howard P. Baden


Book ID
118897246
Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
355 KB
Volume
18
Category
Article
ISSN
0022-1759

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A new technique, temperature gradient gel electrophoresis (TGGE), was applied to the study of heat-induced protein denaturation. The gels used contained 30 mΠΌ Borax \(+75 \mathrm{~mm}\) boric acid, \(\mathrm{pH}\) 8.4, and various concentrations of urea. When this technique was applied to bovine ser