The use of chromatography paper in the study of sterol adduct formation

✍ Scribed by J. W. A. Meijer; C. J. F. Böttcher

• Publisher: Elsevier Science
• Year: 2010
• Tongue: English
• Weight: 382 KB
• Volume: 78
• Category: Article
• ISSN: 0165-0513
• DOI: 10.1002/recl.19590780810

✦ Synopsis

Abstract

In order to investigate the adduct‐forming properties of sterols with very small quantities of these compounds a method has been developed which uses acetylated chromatography paper as the medium in which the adduct can be formed.

For the adduct formation of· cholesterol the results appear to be in agreement with those obtained on a larger scale by refluxing a solution of the sterol in the presence of the other component. The method is also suitable for the investigation of the adduct formation of sterols other than cholesterol.

The relative humidity of the atmosphere in which the reaction takes place has an important influence on the adduct formation.

Two colour reagents for detecting steroids on acetylated paper are described: a solution of benzenesulphonic acid in ether‐methanol, and a solution of antimony trichloride in tetrahydrofurane.