The use of chaotropic salts for separation of ribonucleic acids and proteins from yeast nucleoproteins

Two-dimensional electrophoretic separati

Two-dimensional electrophoretic separation of yeast proteins using a non-linear wide range (pH 3–10) immobilized pH gradient in the first dimension; reproducibility and evidence for isoelectric focusing of alkaline (pI >7) proteins

✍ Norbeck, Joakim; Blomberg, Anders 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 346 KB 👁 2 views

The proteome of the yeast Saccharomyces cerevisiae was analysed by two-dimensional (2D) polyacrylamide gel electrophoresis utilizing a non-linear immobilized pH gradient (3-10) in the first-dimensional separation. Cells were labelled by [ 35 S]methionine incorporation in the respiro-fermentative pha