The use of capillary electrophoresis for the determination of hemoglobin variants
โ Scribed by Prof. Massimo Castagnola; Irene Messana; Loredana Cassiano; Rita Rabino; Diana V. Rossetti; Bruno Giardina
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 610 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
โฆ Synopsis
The use of capillary electrophoresis for the determination of hemoglobin variants
The application of capillary electrophoresis and related techniques for the detection of hemoglobin variants is described. Capillary zone electrophoresis (CZE) was applied for the analysis of intact tetrameric hemoglobin. CZE under denaturing conditions was used for the separation of globin chains. Both CZE and micellar electrokinetic capillary chromatography were applied for a fast and sensitive separation of tryptic digests and for the analysis of amino acid derivatives.
๐ SIMILAR VOLUMES
Capillary affinity electrophoresis was used to study the interactions of lectins from Tetragonolobus purpureas with L-(-)-fucose 1-phosphate. The electrophoretic mobilities of three lectin peaks were influenced by the complexation with the negatively charged fucose derivative. A model that allows th