The use of biotin–avidin binding to facilitate biomodification of thermoresponsive culture surfaces

✍ Scribed by Masanori Nishi; Jun Kobayashi; Sebastian Pechmann; Masayuki Yamato; Yoshikatsu Akiyama; Akihiko Kikuchi; Katsumi Uchida; Marcus Textor; Hirofumi Yajima; Teruo Okano

• Publisher: Elsevier Science
• Year: 2007
• Tongue: English
• Weight: 425 KB
• Volume: 28
• Category: Article
• ISSN: 0142-9612
• DOI: 10.1016/j.biomaterials.2007.08.027

✦ Synopsis

Here, we report biomodification of temperature-responsive culture surfaces with biotinylated biomolecules utilizing streptavidin and biotinylation of the surfaces. Poly(N-isopropylacrylamide-co-2-carboxyisopropylacrylamide) was covalently grafted onto tissue culture polystyrene (TCPS) dishes. Biotinylated Arg-Gly-Asp-Ser (RGDS) peptides with different spacer lengths (biotin-conjugated G n RGDS (n ¼ 1, 6, 12, 16)) were examined. Human umbilical vein endothelial cells (HUVECs) adhered and were well spread on G 12 RGDSimmobilized surfaces in the absence of serum at 37 1C, while much less cell adhesion was observed with the other peptides. Adhered HUVECs were detached on reducing temperature to 20 1C, or on adding free RGDS peptide. Interestingly, cell detachment was accelerated by applying both these techniques. Consequently, by optimizing the spacer length, biomolecules can be functionally immobilized onto thermoresponsive surfaces via the affinity binding between avidin and biotin.