The use of antibiotics in the culture of non-sterile plant protoplasts

✍ Scribed by J. W. Watts; Janet M. King

• Publisher: Springer-Verlag
• Year: 1973
• Tongue: English
• Weight: 361 KB
• Volume: 113
• Category: Article
• ISSN: 0032-0935
• DOI: 10.1007/bf00390514

✦ Synopsis

The use of antibiotics to control infections in cultures of protoplasts of leaf mesophyll cells has been examined. The antifungal agents nystatin and amphotericin B were non-toxic to protoplasts at concentrations that controlled fungal growth (25 units and 2.5 ~g/ml respectively). Of the antibacterial agents examined, only carbenicillin and, to a lesser extent, gentamicin were active against the bacteria usually encountered whilst still permitting normal protoplast metabolism and regeneration. The most satisfactory control of contaminating microorganisms was obtained with a combination of nystatin (25 units/ml) or amphotericin B (2.5 ~g/ml) and carbenicillin (250 ~zg/ml).