The use of anti-immunoglobulin for the immunoprecipitation of labeled proteins from tissue homogenates

✍ Scribed by Amnon Hizi; Gad Yagil

• Book ID: 102984173
• Publisher: Elsevier Science
• Year: 1974
• Tongue: English
• Weight: 829 KB
• Volume: 62
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(74)90171-7

✦ Synopsis

An improved immunochemical procedure for the quantitative isolation of labeled minor proteins from tissue homogenates is worked out and is applied to the isolation of glucose&-phosphate dehydrogenase from mouse liver. Goat anti-enzyme serum is used as primary reagent, followed by rabbit anti-goat IgG, and not by carrier enzyme as in currently used methods. The resulting immunoprecipitates are analyzed by acrylamide gel electrophoresis, so that only counts in enzyme bands are registered. An equivalent precipitate formed with serum from nonimmunized goat serves as an efficient control for coprecipitation.